Service: determination of the prebiotic capacity of food or its components in an in vivo model of C57BL/6 mice. Animal trial with a prebiotic diet and a control diet. Determination of the animals’ general condition, weight, food and water consumption, cecal content, blood for biochemical studies, fecal matter, DNA extraction, and microbiota metagenomic analysis.
Intended users: food laboratories and industries
Methodology: C57BL/6 or balb/c mice are used. The animals have unlimited access to both water and food and will be kept at a constant temperature (22 °C +1) and humidity, along a 12h light/12h dark cycle.
Experimental Design – Group 1 control: standard diet. Group 2 positive control: standard diet supplemented with inulin dose based on the reference daily intake (RDI). Group 3 diet with the sample to be evaluated: standard diet supplemented with the food or ingredient to be evaluated.
Determination of: 1- general condition of the animals, 2- weight, 3- food consumption, 4- water consumption, 5- collection of cecal content, blood collection, macroscopic observation of organs, determination of weight and preservation of liver, small and large intestine, cecum, spleen and stomach. Biochemical determination of total cholesterol, triglycerides, HDL, LDL, glucose, cortisol, IgG and liver enzymes GOT, FAL and GPT. 6- Collection of fecal matter and extraction of total DNA . 7- Fecal microbiota metagenomic analysis, including 2 samples per animal (time 0 and final time)
A technical report is delivered. The service is associated with the CONICET’s STAN ST5397.
Lead professionals: Mara Mattalloni, Gabriela Pérez, Emiliano Salvucci